Electrochemical determination of hydrogen peroxide using o -dianisidine as substrate and hemoglobin as catalyst |
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Authors: | Wei Sun Hong Jiang and Kui Jiao |
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Institution: | (1) College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 266042 Qingdao, PR China |
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Abstract: | A new electrochemical method for the determination of microamounts of hydrogen peroxide utilizing o-dianisidine (ODA) as substrate
and hemoglobin (Hb) as catalyst is described in this paper. Hb can be used as mimetic peroxidase and it can catalyse the reduction
of hydrogen peroxide with the subsequent oxidation of ODA. The oxidative reaction product is an azo compound, which is an
electroactive substance and has a sensitive second-order derivative polarographic reductive peak at the potential of -0.58
V (vs. SCE) in pH 80 Britton-Robinson (B-R) buffer solution. The conditions of Hb-catalytic reaction and polarographic detection
of the reaction product were carefully studied. By using this polarographic peak and under optimal conditions, the calibration
curve for the H2O2 was constructed in the linear range of 2.0 x 10-7 ∼ 10 x 10-4 mol/l with the detection limit of 5.0 x 10-8 mol/l. This method can also be used to the determination of Hb content in the range of 20 x 10-9 ∼ 30 x 10-7 mol/l with a detection limit of 10 x 10-9 mol/l. The proposed method was further applied to the determination of the content of H2O2 in fresh rainwater with satisfactory results. The catalytic reaction mechanism and the electrode reductive process of the
reaction product were carefully studied. |
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Keywords: | Hemoglobin hydrogen peroxide o-dianisidine polarography |
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