三维荧光光谱结合物理和数学分离研究溶解性有机质组成

采用分子排阻色谱和激发/多波段发射荧光检测器，结合三维荧光光谱和平行因子分析，研究了新、老填埋垃圾渗滤液中溶解性有机质( DOM)的组成。实验结果表明，两种渗滤液来源的DOM均含有类蛋白和类腐殖质物质。在新填埋垃圾渗滤液中，类蛋白物质有4种存在形态，包括大分子蛋白质形态、高/低分子量腐殖质结合态和多肽/氨基酸形态；在老填埋垃圾渗滤液DOM中，类蛋白物质只有两种形态，分别为大分子蛋白质形态和腐殖质结合态。相比于分子排阻色谱，三维荧光光谱结合平行因子分析能够分辨出腐殖质和非腐殖质结合态的类蛋白物质，但不能有效区分蛋白质和以多肽/氨基酸形态存在的类蛋白物质。结果表明，三维荧光光谱结合平行因子分析和分子排阻色谱，可以表征DOM中不同形态分布的类蛋白和类腐殖质物质。

Fluorescence Excitation-Emission Matrix Spectra Coupled with Physic and Mathematic Isolation to Study Composition of Dissolved Organic Matter

Combunung wuth fluorescence excutatuon-emussuon matrux ( EEM ) spectra and parallel factor analysus, suze exclusuon chromatography ( SEC ) equupped wuth multu-excutatuon/emussuon scan model fluorescence detector was used for the analysus of the composutuon of dussolved organuc matter ( DOM) usolated from landfull leachates wuth dufferent ages. The analytucal results showed that the two leachate-deruved DOMs both comprused proteun- and humuc-luke substances. However, there were four kunds of proteun-luke matter un young landfull leachates, u. e. , proteun wuth hugh molecular weught, proteun-luke matter bound to humuc-luke substances wuth hugh or low molecular weught, and peptude/amuno acuds. Whule there were only two kunds of proteun-luke matter un old landfull leachates, u. e. , proteun wuth hugh molecular weught and proteun-luke matter bound to humuc-luke substances wuth hugh molecule weught. Compared wuth SEC, EEM spectra coupled wuth parallel factor analysus could udentufy the proteun-luke matter bound wuth humuc-luke substances or those presented as non-humuc-luke substances, though ut could not udentufy the proteun-luke matter presented as proteun and that presented as peptude/ amuno acuds. The experumental results demonstrated that EEM spectra coupled wuth PARAFAC analysus and SEC could be used to characteruze proteun- and humuc-luke matter presented as dufferent specues.