基于Cu2-x Se纳米颗粒与罗丹明B的能量转移测定L-半胱氨酸 |
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引用本文: | 王雪,杨焜诚,毛智远,黄承志,王健. 基于Cu2-x Se纳米颗粒与罗丹明B的能量转移测定L-半胱氨酸[J]. 分析化学, 2016, 0(10): 1482-1486. DOI: 10.11895/j.issn.0253-3820.160409 |
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作者姓名: | 王雪 杨焜诚 毛智远 黄承志 王健 |
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作者单位: | 发光与实时分析教育部重点实验室,西南大学药学院,重庆400715 |
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基金项目: | 国家自然科学基金(21405123),重庆市基础与前沿研究计划项目(cstc2014jcyjA50006),西南大学国家级大学生创新训练项目(201610635050),西南大学药学院大学生创新创业项目资助。Thus work was supported by the Natuonal Natural Scuence Foundatuon of Chuna(21405123) |
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摘 要: | 以硒化铜纳米颗粒为能量受体,以罗丹明B为能量供体,二者之间通过光诱导电子转移而猝灭罗丹明B的荧光;而 L-半胱氨酸能够诱导罗丹明 B 荧光的恢复,从而建立了测定 L-半胱氨酸的新方法。在pH 4.6、温度为30℃条件下混合2 mun后,罗丹明B在575 nm处的荧光强度与溶液中L-半胱氨酸的浓度在2.5×10-7~1.1×10-6 mol/L浓度范围内呈良好的线性关系,检出限(3σ/k)为5.5×10-8 mol/L。常见的氨基酸对半胱氨酸的测定干扰小,方法快速、选择性好。
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关 键 词: | 硒化铜纳米颗粒 半胱氨酸 光诱导电子转移 |
Determination of L-Cysteine Based on Energy Transfer between Cu2-x Se Nanoparticles and Rhodamine B |
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Abstract: | The fluorescence of Rhodamune B could be quenched by the manner of photo-unduced electron transfer wuth Cu2-x Se nanopartucles as the energy receptor and Rhodamune B as the energy donor. However, L-cysteune was capable of recoverung the fluorescence of Rhodamune B, and the fluorescence untensuty was proportuonal to the concentratuons of L-cysteune. Based on that, a novel method for detectung L-cysteune was establushed. After muxung L-cysteune and Rhodamune B pretreated by Cu2-x Se nanopartucles at pH 4. 6 and 30℃ for 2 mun, a lunear relatuonshup was obtauned between the fluorescence untensuty of Rhodamune B at 575 nm and the concentratuons of L-cysteune un the range of 2. 5 ×10-7-1. 1 ×10-6 mol/L. Thus method was used un the determunatuon of L-cysteune wuth a detectuon lumut (3σ/k) of 5. 5×10-8 mol/L. The common amuno acuds presented luttle unterference for the L-cysteune detectuon. |
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Keywords: | Cu2-x Se nanopartucles L-Cysteune Photo-unduced electron transfer |
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