显微荧光光谱原位测定红树植物根表面微区中的蒽 |
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引用本文: | 谭华东,李锐龙,朱亚先,张勇. 显微荧光光谱原位测定红树植物根表面微区中的蒽[J]. 分析化学, 2016, 0(8): 1281-1285. DOI: 10.11895/j.issn.0253-3820.160049 |
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作者姓名: | 谭华东 李锐龙 朱亚先 张勇 |
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作者单位: | 1. 厦门大学环境与生态学院,近海海洋环境科学国家重点实验室,厦门361102;2. 厦门大学化学与化工学院,厦门,361005 |
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基金项目: | 国家自然科学基金(.21577110;21177102),高等学校博士学科点专项科研基金(20130121130005) |
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摘 要: | 自行搭建显微荧光光谱分析( Microscopic fluorescence spectrometric analysis, MFSA)系统,并将其用于吸附于红树植物秋茄( Kandelia obovata, K. obovata)和白骨壤( Avicennia marina, A. marina)根表面微区中蒽( Anthrancene, Ant)的原位测定。在同步扫描模式下,以波长差为60 nm,吸附于K. obovata和A. marina根表面微区中Ant(5.3 pg/μm2)的荧光信号有最大信噪比(5.5和6.8)。所建方法的线性范围分别为5.3 ~63.2 pg/μm2和10.5~52.6 pg/μm2,检出限分别为1.1和5.5 pg/μm2,相对标准偏差均小于12.5%(n=9),加标回收率分别为98.1%~117.0%和81.2%~110.9%。结果表明,搭建的MFSA系统具备原位获取植物根表面微区Ant荧光光谱定量信息和荧光图像信息的能力。
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关 键 词: | 显微荧光光谱分析 蒽 根表面微区 原位 |
In situ Determination of Anthracene Adsorbed onto Mangrove Root Surface Micro-zone Using Microscopic Fluorescence Spectrometric Analysis System |
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Abstract: | A microscopic fluorescence spectrometric analysis ( MFSA) system was set in the laboratory. A novel method for in situ determination of Anthracene ( Ant) adsorbed onto root surface micro-zone of two kinks of mangrove plant, named Kandelia obovata ( K. obovata) and Avicennia marina ( A. marina) by MFSA was established. Fluorescence spectra of Ant adsorbed on root surface micro-zone were obtained by synchronous scanning mode. The signal to noise (S/N) of Ant (5. 3 pg/μm2) adsorbed on K. obovata and A. marina root surface micro-zone increased up to 5. 5 and 6. 8 while wavelength offset (△λ) both were at 60 nm, respectively. The linear dynamic ranges of established method were 5. 3-63. 2 pg/μm2 for K. obovata and 10. 5-52. 6 pg/μm2 for A. marina, with the detection limits of 1. 1 pg/μm2 and 5. 5 pg/μm2 , respectively. The relative standard deviations were both less than 12 . 5% ( n=9 ) , and the recoveries were 98 . 1% -117. 0% and 81. 2%-110. 9%, respectively. The result showed that the MFSA system had ability to obtain quantitative information of fluorescence spectra and fluorescence image of Ant adsorbed onto plant roots surface micro-zone. |
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Keywords: | Microscopic fluorescence spectrometric analysis Anthracene Root surface micro-zone in situ |
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