| 比率型荧光探针结合先进校正模型用于环境水样中亚硝酸根离子的定量分析 |
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| 引用本文： | 崔银银,陈增萍,严秀芳,俞汝勤.比率型荧光探针结合先进校正模型用于环境水样中亚硝酸根离子的定量分析[J].分析化学,2016(8):1250-1256. |
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| 作者姓名： | 崔银银 陈增萍 严秀芳 俞汝勤 |
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| 作者单位： | 化学生物传感与计量学国家重点实验室，湖南大学化学化工学院，长沙410082 |
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| 基金项目： | 国家自然科学基金(.21275046;21475039)，国家重大科学仪器设备开发专项(2011YQ0301240102)，湖南省自然科学基金(2015JJ2028)，教育部博士点基金(20130161110027)，新世纪优秀人才支持计划(NCET-12-0161) |
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| 摘 要： | 将新型荧光光谱定量分析模型与比率型荧光探针2,3-二氨基萘相结合,发展了一种用于水溶液中亚硝酸根离子( NO-2)定量分析的新方法；并考察了本方法对含有散射物质和吸光物质的浑浊水样中NO-2进行直接定量分析的性能。结果表明,本方法对实际浑浊环境水样中NO-2的检出限和定量下限分别为1.9和5.8 nmol/L,其定量分析结果的回收率在90.8%~103%之间,与高效液相色谱-二极管阵列检测器联用仪的定量分析结果的回收率没有显著性差异。
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| 关 键 词： | 比率型荧光探针 亚硝酸根 定量分析 荧光定量模型 |
Quantification of Nitrite Ion in Environmental Water Samples by Ratiometric Fluorescence Indicator in Combination with Quantitative Fluorescence Model |
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| Abstract: | A novel method was proposed for the quantification of nitrite ion in aqueous samples by the combination of an advanced quantitative fluorescence model with ratiometric fluorescence indicator 2 , 3-diaminonaphthalene . The proposed method was tentatively applied to direct quantitative determination of nitrite ion in turbid environmental water samples containing both scatterers and absorbers; and its performance was thoroughly investigated and evaluated. Experimental results showed that the proposed method could realize accurate quantitative determination of nitrite ion in environmental water samples with recovery rates in the range of 90. 8%-103. 0%, which were comparable to the corresponding values of HPLC-DAD experiments. The limit of detection and limit of quantification of the proposed method were estimated to be 1 . 9 and 5. 8 nmo/L, respectively. |
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| Keywords: | Ratiomatric fluorescence indicator Nitrite ion Quantitative analysis Quantitative fluorescence model |
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