| PLANT REGENERATION FROM IMMATURE COTYLEDONOUS TISSUE CULTURES OF SOYBEAN BY SOMATIC EMBRYOGENESIS |
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| 引用本文： | 冯新华,蒋兴村,邵启全. PLANT REGENERATION FROM IMMATURE COTYLEDONOUS TISSUE CULTURES OF SOYBEAN BY SOMATIC EMBRYOGENESIS[J]. 中国科学B辑(英文版), 1989, 0(1) |
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| 作者姓名： | 冯新华 蒋兴村 邵启全 |
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| 作者单位： | Institute of Genetics，Academia Sinica，Beijing，Institute of Genetics，Academia Sinica，Beijing，Institute of Genetics，Academia Sinica，Beijing |
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| 摘 要： | Genetic engineering of soybean is limited because of the difficulty of regeneration of plants via in vitro culture. Here we report that a method of obtaining somatic embryos and regenerated plants of soybean has been developed. It is achieved by culturing immature cotyledons of soybean on modified Murashige-Skoog medium supplemented with high concentration of either napthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). High induction frequencies of 85% and 94% are reached by using 10mg/l NAA and 5mg/l 2,4-D, respectively. A number of regenerated plantlets of soybean are transplanted to sterilized soil and grown into 15 full plants in pots.
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PLANT REGENERATION FROM IMMATURE COTYLEDONOUS TISSUE CULTURES OF SOYBEAN BY SOMATIC EMBRYOGENESIS |
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| FENG XIN-HUA JIANG XING-CUN AND SHAO QI-QUAN. PLANT REGENERATION FROM IMMATURE COTYLEDONOUS TISSUE CULTURES OF SOYBEAN BY SOMATIC EMBRYOGENESIS[J]. Science in China(Chemistry), 1989, 0(1) |
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| Authors: | FENG XIN-HUA JIANG XING-CUN AND SHAO QI-QUAN |
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| Abstract: | Genetic engineering of soybean is limited because of the difficulty of regeneration of plants via in vitro culture. Here we report that a method of obtaining somatic embryos and regenerated plants of soybean has been developed. It is achieved by culturing immature cotyledons of soybean on modified Murashige-Skoog medium supplemented with high concentration of either napthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). High induction frequencies of 85% and 94% are reached by using 10mg/l NAA and 5mg/l 2,4-D, respectively. A number of regenerated plantlets of soybean are transplanted to sterilized soil and grown into 15 full plants in pots. |
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| Keywords: | soybean regeneration cotyledonous tissues somatic embryogenesis. |
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