Neurite outgrowth-promoting active constituents of the Japanese cypress (Chamaecyparis obtusa) |
| |
Authors: | Kuroyanagi Masanori Ikeda Ryuya Gao Hui Yuan Muto Norio Otaki Keisuke Sano Toshikazu Kawahara Nobuo Nakane Takahisa |
| |
Affiliation: | Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Hiroshima, Japan. kuroyang@pu-hiroshim.ac.jp |
| |
Abstract: | In the screening of biologically active constituents from woody plants, the methanol extract of leaves of Chamaecyparis obtusa showed potent neurite outgrowth-promoting activity in neuronal PC12 cells. The ethyl acetate-soluble fraction of the methanol extract showed potent activity and was separated by means of various chromatographic methods to give the two new compounds 1 and 2, as well as 11 known lignan and sesquiterpene derivatives. The structures of the new compounds were determined to be 9-O-acetyldihydrosesamin (1) and 9-O-(11-hydroxyeudesman-4-yl)dihydrosesamin (2), respectively, in NMR studies including 2D-NMR experiments. Of the 13 compounds, the known compound hinokinin (5) and the new compound 2 showed potent neurite outgrowth-promoting activity in PC 12 cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|