| 中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离 |
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| 引用本文： | 田淑芳,邹雪艳,卢海涛,何建英,陈丹云,赵彦保,郭静玉. 中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离[J]. 无机化学学报, 2013, 29(18) |
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| 作者姓名： | 田淑芳 邹雪艳 卢海涛 何建英 陈丹云 赵彦保 郭静玉 |
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| 作者单位： | 河南大学化学化工学院, 开封 475004;河南大学特种功能材料重点实验室, 开封 475004,河南大学特种功能材料重点实验室, 开封 475004,河南大学民生学院, 开封 475004,河南大学化学化工学院, 开封 475004,河南大学化学化工学院, 开封 475004,河南大学特种功能材料重点实验室, 开封 475004,河南大学化学化工学院, 开封 475004 |
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| 基金项目： | 河南省教育厅科学技术重点研究项目(No.14B150003), 国家自然科学基金(No.21271062)资助项目。 |
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| 摘 要： | 利用水热法合成了中空巯基纳米二氧化硅微球(SiO2-SH), 然后在其表面修饰亚氨基二乙酸基团(-IDA), 形成了中空SiO2-SH/IDA双功能化纳米微球。利用该纳米微球表面的-SH和-IDA双功能团, 可以更多的吸附溶液中的Ni2+, 形成SiO2-SH/IDA-Ni2+复合微球从而可以更好的分离以六聚组氨酸为标签的(His-tagged)蛋白。结果显示制备的样品对分离His-tagged蛋白具有广谱性, 并且具有较好的再生能力。
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| 关 键 词： | 中空微球 SiO2 蛋白分离 功能化 亲和 |
Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein |
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| TIAN Shu-Fang,ZOU Xue-Yan,LU Hai-Tao,HE Jian-Ying,CHEN Dan-Yun,ZHAO Yan-Bao and GUO Jing-Yu. Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein[J]. Chinese Journal of Inorganic Chemistry, 2013, 29(18) |
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| Authors: | TIAN Shu-Fang ZOU Xue-Yan LU Hai-Tao HE Jian-Ying CHEN Dan-Yun ZHAO Yan-Bao GUO Jing-Yu |
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| Affiliation: | College of Chemistry and Chemical Enginerring, Henan University, Kaifeng, Henan 475004, China;Key Laboratory for Special Functional Materials, Henan University, Kaifeng, Henan 475004, China,Key Laboratory for Special Functional Materials, Henan University, Kaifeng, Henan 475004, China,Henan University Minsheng College, Kaifeng, Henan 475004, China,College of Chemistry and Chemical Enginerring, Henan University, Kaifeng, Henan 475004, China,College of Chemistry and Chemical Enginerring, Henan University, Kaifeng, Henan 475004, China,Key Laboratory for Special Functional Materials, Henan University, Kaifeng, Henan 475004, China and College of Chemistry and Chemical Enginerring, Henan University, Kaifeng, Henan 475004, China |
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| Abstract: | Hollow silica nanospheres with thiol groups (SiO2-SH NSs) have been successfully prepared through a hydrothermal route. Then the SiO2-SH NSs was further modified by conjugating iminodiacetic acid (IDA) to bear dual chelating groups (-SH and -COOH). After chelating Ni2+, these hollow NSs with dual chelating groups were applied to purify histidine-tagged (His-tagged) proteins. Results show that these hollow NSs can be widely used to separate His-tagged proteins and have a good reused ability. |
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| Keywords: | hollow microspheres silica separation of protein functionalization affinity |
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