Detailed Structural Analysis of the Immunoregulatory Polysaccharides from the Mycobacterium Bovis BCG |
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| Authors: | Lan Luo Xuemei Song Xiao Chang Sheng Huang Yunxi Zhou Shengmei Yang Yan Zhu Lanyan Zhang Yongsheng Wu Junyin Zhang Zhipeng Zhou Mingyi Wu |
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| Affiliation: | 1.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Hunan Jiuzhitang Siqi Biological Pharmaceutical Co., Ltd., Changsha 410329, China;4.Jiuzhitang Co., Ltd., Changsha 410205, China |
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| Abstract: | Bacillus Calmette-Guérin polysaccharide and nucleic acid (BCG-PSN), extracted from Mycobacterium bovis, is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an α-D-(1→4)-mannan with (1→2)-linked branches, and an α-D-(1→4)-glucan with (1→6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-α, IL-6, IL-1β, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN. |
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| Keywords: | active ingredients polysaccharide purification glucan mannan immunomodulator |
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