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Sexing Bovine Embryos Using PCR Amplification of Bovine SRY Sequence
作者姓名:曾溢滔  张美兰  陈美珏  周霞娣  黄英  任兆瑞  黄淑帧  胡明信  吴学清  高建明  张斌  徐慧如
作者单位:Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC),Department of Animal Husbandry,Beijing Agriculture College,Beijing 102208,PRC,Department of Animal Husbandry,Beijing Agriculture College,Beijing 102208,PRC,Department of Animal Husbandry,Beijing Agriculture College,Beijing 102208,PRC,Department of Animal Husbandry,Beijing Agriculture College,Beijing 102208,PRC,Department of Animal Husbandry,Beijing Agriculture College,Beijing 102208,PRC
基金项目:Project supported by the National Natural Science Foundation of China.
摘    要:This study analyses the bovine SRY DNA sequence by direct sequencing procedure, followed by the designation of the PCR primers specific for bovine SRY. Using PCR amplification of bovine SRY gene, the embryo sex was determined. The results of the embryo sex identification were confirmed after the embryo transfer and pregnancies.


Sexing Bovine Embryos Using PCR Amplification of Bovine SRY Sequence
ZENG Yi-Tao ZHANG Mei-Lan,CHEN Mei-JueZHOU Xia-Di,HUANG Ying Ren Zhao-RuiHuang Shu-Zhen.Sexing Bovine Embryos Using PCR Amplification of Bovine SRY Sequence[J].Science in China(Chemistry),1994(2).
Authors:ZENG Yi-Tao ZHANG Mei-Lan  CHEN Mei-JueZHOU Xia-Di  HUANG Ying Ren Zhao-RuiHuang Shu-Zhen
Abstract:This study analyses the bovine SRY DNA sequence by direct sequencing procedure, followed by the designation of the PCR primers specific for bovine SRY. Using PCR amplification of bovine SRY gene, the embryo sex was determined. The results of the embryo sex identification were confirmed after the embryo transfer and pregnancies.
Keywords:bovine  embryo  sex determination  PCR  SRY  
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