毛细管电泳免疫化学发光检测鼠血管平滑肌细胞中zmol骨形成蛋白-2

近年来，结合毛细管电泳的免疫分析研究在不断加强.特别是毛细管电泳免疫激光诱导荧光(CEIA-LIF)检测由于具有较高的灵敏度而十分引人注目.常文保等用CEIA-LIF检测雌三醇，检出限为31.6ng/L，可用于血清和尿样分析.Kennedy等用芯片CEIA-LIF检测了鼠胰腺细胞中的胰岛素，

Capillary Electrophoresis Immunoassay Chemiluminescence Detection of zmol Bone Morphogenic Protein-2 in Rat Vascular Smooth Muscle Cells

In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H2O2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab6-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.