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毛细管电泳免疫化学发光检测鼠血管平滑肌细胞中zmol骨形成蛋白-2
引用本文:王军华,黄卫华,刘彦明,程介克.毛细管电泳免疫化学发光检测鼠血管平滑肌细胞中zmol骨形成蛋白-2[J].高等学校化学学报,2004,25(9):1642-1644.
作者姓名:王军华  黄卫华  刘彦明  程介克
作者单位:武汉大学化学与分子科学学院,武汉,430072;河南信阳师范学院化学化工学院,信阳,464000
基金项目:国家自然科学基金 (批准号 :2 0 2 990 3 4)资助
摘    要:近年来,结合毛细管电泳的免疫分析研究在不断加强.特别是毛细管电泳免疫激光诱导荧光(CEIA-LIF)检测由于具有较高的灵敏度而十分引人注目.常文保等用CEIA-LIF检测雌三醇,检出限为31.6ng/L,可用于血清和尿样分析.Kennedy等用芯片CEIA-LIF检测了鼠胰腺细胞中的胰岛素,

关 键 词:毛细管电泳  化学发光  免疫分析  抗原抗体  骨形成蛋白-2
文章编号:0251-0790(2004)09-1642-03
收稿时间:2004-03-04

Capillary Electrophoresis Immunoassay Chemiluminescence Detection of zmol Bone Morphogenic Protein-2 in Rat Vascular Smooth Muscle Cells
WANG Jun-Hua,HUANG Wei-Hua,LIU Yan-Ming,CHENG Jie-Ke.Capillary Electrophoresis Immunoassay Chemiluminescence Detection of zmol Bone Morphogenic Protein-2 in Rat Vascular Smooth Muscle Cells[J].Chemical Research In Chinese Universities,2004,25(9):1642-1644.
Authors:WANG Jun-Hua  HUANG Wei-Hua  LIU Yan-Ming  CHENG Jie-Ke
Institution:WANG Jun-Hua1,HUANG Wei-Hua1,LIU Yan-Ming2,CHENG Jie-Ke 1 *
Abstract:In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H2O2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab6-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.
Keywords:Capillary electrophoresis  Chemiluminescence  Immunoassay  Antigen antibody  Bone morphogenic protein-2
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