| Abstract: | The present study focuses on designing and fabricating an electrochemical aptasensor for the label free detection of bisphenol A (BPA) using gold nanoparticles (Au NPs) immobilized on functional cupper magnetic nanoparticles (CuFe2O4‐SH) and multiwall carbon nanotubes (MWCNTs) modified with aptamer and 6‐mercapto‐1‐hexanol (MCH). A number of analysis techniques were used to characterize the nanocomposite, including Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometer, elemental mapping analysis and energy dispersive x‐ray diffraction. The results of the analyses revealed that the fabricated aptasensor had an acceptable linearity index (0.05‐9 nM) with an ultralow detection limit (25.2 pM) when used to determine BPA. Electrochemical experiments were conducted using a Fe(CN)6]3?/Fe(CN)6]4? redox system. The results of the electrochemical tests indicated that the existence of Au NPs along with magnetic nanoparticles and MWCNTs in nanocomposite led to a synergistic augmentation on the surface of the modified electrode, thus facilitating the efficient sensing of BPA. This method is highly selective, sensitive and environmentally friendly. Moreover, proposed aptasensor has valuable potential applications in medical diagnostics and food industries where a fast and reliable detection of BPA is of paramount importance for the health of the public. |
