Rapid microarray processing using a disposable hybridization chamber with an integrated micropump |
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Authors: | Rupp Jochen Schmidt Manuela Münch Susanne Cavalar Markus Steller Ulf Steigert Jürgen Stumber Michael Dorrer Christian Rothacher Peter Zengerle Roland Daub Martina |
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Institution: | Robert Bosch GmbH, Corporate Sector Research Microsystem Technologies, Robert Bosch Platz 1, 70839 Gerlingen, Germany. Jochen.Rupp3@de.bosch.com |
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Abstract: | We present a disposable microarray hybridization chamber with an integrated micropump to speed up diffusion based reaction kinetics by generating convective flow. The time-to-result for the hybridization reaction was reduced from 60 min (standard protocol) down to 15 min for a commercially available microarray. The integrated displacement micropump is pneumatically actuated. It includes two active microvalves and is designed for low-cost, high volume manufacturing. The setup is made out of two microstructured polymer parts realized in polycarbonate (PC) separated by a 25 μm thermoplastic elastomer (TPE) membrane. Pump rate can be controlled between 0.3 μl s(-1) and 5.7 μl s(-1) at actuation frequencies between 0.2 Hz and 8.0 Hz, respectively. |
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