Langmuir monolayers and Langmuir–Blodgett films of ferritin prepared by using a surfactant mixture of eicosylamine (EA) and methyl stearate (SME)

Magnetic Langmuir–Blodgett films of ferritin have been prepared by using the adsorption properties of a 1/4 mixed monolayer of eicosylamine (EA) and methyl stearate (SME). BAM images show that a more homogeneous distribution of ferritin at the air–water interface is achieved by using this mixture of surfactants instead of the DODA/SME mixed matrix of a previous work. Transfer of the monolayer onto different substrates allowed the preparation of multilayer LB films. Infrared and UV–Vis spectroscopies indicate that ferritin molecules are incorporated within the LB films. Furthermore, UV–Vis spectroscopy measurements reveal that the amount of ferritin incorporated into these LB film has been increased with respect to that in the DODA/SME LB films. Finally magnetic measurements confirm that the superparamagnetic properties of this molecule are preserved in the LB films.