Incorporation of carboxylation multiwalled carbon nanotubes into biodegradable poly(lactic-co-glycolic acid) for bone tissue engineering |
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Authors: | Lin Cuilin Wang Yifang Lai Youqun Yang Wei Jiao Fei Zhang Honggang Ye Shefang Zhang Qiqing |
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Affiliation: | a Research Center of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen University, Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering Technology of Xiamen City, Xiamen 361005, PR China;b Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, PR China |
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Abstract: | Biodegradable poly(lactic-co-glycolic acid) (PLGA)/carboxyl-functionalized multi-walled carbon nanotube (c-MWCNT) nanocomposites were successfully prepared via solvent casting technique. Rat bone marrow-derived mesenchymal stem cells (MSCs) were employed to assess the biocompatibility of the nanocomposites in vitro. Scanning electron microscopy (SEM) observations revealed that c-MWCNTs gave a better dispersion than unmodified MWCNTs in the PLGA matrix. Surface properties were determined by means of static contact angle, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) analysis. The presence of c-MWCNTs increased the mechanical properties of the nanocomposites. Seven-week period in vitro degradation test showed the addition of c-MWCNTs accelerated the hydrolytic degradation of PLGA. In addition, SEM proved that the cells could adhere to and spread on films via cytoplasmic processes. Compared with control groups, MSCs cultured onto PLGA/c-MWCNT nanocomposites exhibited better adhesion and viability and also displayed significantly higher production levels of alkaline phosphatase (ALP) over 21 days culture. These results demonstrated that c-MWCNTs modified PLGA films were beneficial for promoting cell growth and inducing MSCs to differentiate into osteoblasts. This work presented here had potential applications in the development of 3-D scaffolds for bone tissue engineering. |
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Keywords: | Poly(lactic-co-glycolic acid) Multiwalled carbon nanotube Mesenchymal stem cells Nanocomposites |
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