一种新型C18酯型反相高效液相色谱填料的制备

 发展了一种用于制备反相高效液相色谱填料的实用、经 济的方法。以γ-环氧丙基氧丙基三甲氧基硅烷为中间偶联试剂，将十八烷基脂肪酸 键合至硅胶上。元素分析、色谱测试验证了该工艺的可行性和填料的反相色谱行为。

A Novel Method for Preparation of C18 Ester-BondRP-HPLC Packing

RP-HPLC packings have found widespread application in the fields of biology, chemistry and pharmaceutical industry, etc. C18-bonded phase is one of the most important packing. In order to avoid using toxic and unstable chloro-containing silane as derivatization reagent, a new practical and economic procedure had been developed to prepare RP packing. Octadecyl group was bound onto YQG80, 5 microns silica (Tsingdao Oceanic Chemical Factory, particle size d.p. 5 microns) with gamma-glycidoxypropyltrimethoxysilane as coupling agent. Elemental analysis and chromatographic evaluation confirmed the feasibility of the procedure and RP behavior of the packing. After eluting with 500 column volume of mobile phase at different pH (2, 5 and 8), the column still showed good performance (> 64,000 theoretical plates/m) and stability.