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Thermodynamic study on the interaction of cyanide ion and jack bean urease at different temperatures |
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| Authors: | G Rezaei Behbehani A A Saboury M Mohebbian J Abedini S Tahmasebi Sarvestani |
| Institution: | 1. Chemistry Department, Imam Khomeini International University, Qazvin, Iran 2. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran 3. Chemistry Department, Payame Noor University (PNU), Abhar, Iran |
| Abstract: | A method based on Isothermal Titration Calorimety (ITC) is described for the thermodynamic assay of jack bean urease. Inhibitory activity of cyanide ion was examined against jack bean urease (JBU), at 27 and 37 oC in 30 mM Tris buffer of pH = 7. The binding parameters of the CN? + JBU complexation have been calculated. It was found that in the low and high concentrations of the cyanide ions, the JBU structure was destabilized, resulting in a decrease in its biological activity. |
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