水稻microRNA及其靶基因的系统鉴定

miRNA在植物生长发育和环境胁迫中发挥着重要的作用.本研究基于49个水稻小RNA 测序数据，系统分析了已知miRNA的表达，并鉴定到了新的水稻miRNA.我们发现，miRBase上注释的水稻miRNA中，只有39％具有可检测的表达. 此外，对水稻降解组数据的分析鉴定到一些新的miRNA靶基因，包括两个新的miR444靶基因，它们分别编码WD40蛋白和热休克因子型DNA结合蛋白.

Systematic identification of miRNAs and miRNA targets in Oryza sativa

miRNAs play essential roles in plant development and environmental adaption. Here, based on 49 Oryza sativa (rice) small RNA-seq datasets, we systematically analyzed the expressions of known miRNAs and identified novel miRNAs in rice. We showed that only 39% of annotated miRNAs on miRBase have detectable expressions. Additionally, analysis of rice degradome datasets identified a few new miRNA targets including two miR444 targets that encode a WD40 protein and a HSF (heat shock factor)-type DNA-binding protein, respectively.