基于壳聚糖-溴化氰改性褐藻酸钠凝集作用的日本血吸虫安培免疫传感器

研制了一种基于壳聚糖和溴化氰改性褐藻酸钠凝集作用的日本血吸虫安培免疫传感器.褐藻酸钠-抗体复合物通过静电吸附作用被凝集到含石墨-石蜡-壳聚糖组分的电极表面,然后与抗原和酶标抗原进行竞争反应,以邻氨基酚(o-AP)为电子媒介,通过测定酶催化下双氧水对其氧化的电流大小来间接测定抗原的浓度.研究表明这种免疫传感器具有很低的非特异吸附性能,而且在经过简单的处理后可以重复使用,其重现性和灵敏度良好.传感器对日本血吸虫抗原的响应在0.64～40μg/mL之间呈线性关系.检测限为0.64μg/mL.将电极应用于兔血清中日本血吸虫抗原的测定,取得了满意结果.

A Novel Amperometric Immunosensor Based on Aggregation of Chitosan and CNBr-activated Alginate for Determining Schistosoma Japonicum

Anovel schistosoma japonicum(Sj) imunnosensor based on aggregation of chitosan and CNBr-activated alginate is developed.The complex formed by CNBr-activated alginate and antibody is aggregated on the surface of the paraffin-graphite-chitosan electrode by electrostatic adsorption(coacervation).The concentration of SjAg can be detected by determining the redox current of o-aminophenol oxidized by H2O2in the presence of HRP.The electrode can be reused by simple treatment.Moreover, the imunnosensor shows some improved performances including high sensitivity, selectivity and less non-specific adsorption.The sensor exhibits a linear response to SjAg in the concentration range from 0.64 to 40 μg/mL.The sensor was applied to the determination of SjAg in rabbit serum with satisfactory results.