| Abstract: | Homogenized aliquots (100 g) of the liver (8.4 kg, 5 m?g As g?1) of a tiger shark (Galeocerdo cuvier) were extracted with chloroform/methanol, and the extracts purified by countercurrent extraction (hexane/87% ethanol), silica gel column chromatography (chloroform/methanol mixtures as mobile phases), and silica gel thin-layer chromatography (chloroform/methanol/acetic acid). The purified samples (24 mg arsenic g?1) gave no 31P NMR signal, but gave 1H and 13C NMR signals with similarities to those of dipalmitoylphosphatidic acid and salad on and also signals indicative of the presence of methylated arsenic compounds. The sample could contain a diacyl glyceride with a methylated arsenic group. |
