冬青属苦丁茶不同种质材料的过氧化物酶同工酶分析

应用聚丙烯酰胺凝胶电泳分析了冬青属5种苦丁茶的过氧化物酶同工酶酶谱,并根据同工酶分析结果探讨了冬青属苦丁茶种间的亲缘关系以及种内不同种质材料的遗传差异。研究结果表明,所有不同物种的供试材料均有两条共同的特征谱带,同时又有丰富的酶带多态性。同一物种的不同种质材料的过氧化物酶同工酶酶谱也显现出一定的差异。过氧化物酶同工酶的酶谱分析结果可以作为判定不同物种间的亲缘关系及冬青属苦丁茶种级水平分类的依据之一,但同一物种内不同种质材料的遗传差异以及种下分类群的划分则尚需更多分子生物学及形态学研究结果相互佐证。

STUDY ON PEROXIDASE ISOZYMES OF THE GERMPLASM MATERIALS OF FIVE "KUDINGCHA TEA" SPECIES IN ILEX L.

Peroxidase isoenzymes offive"Kudingcha tea" species in IlexL.were studied by means of polyacrylamid gel electrophoresis.The inter-specific relationship and genetic differences of the various germplasm materials of the same species wereanalyzed based on the comparison of the isoenzyme bands. The results showed that thecommon characteristic isoenzyme bands of the genuscould be observed in all test materials. Moreover, there was rich polymorphismof the isoenzyme bands, simultaneously. The zymograms of peroxidase isoenzyme of variousgermplasm materials of the same species also showed differences in some degrees. The result of zymogram analysis of peroxidase could be considered as one of the bases for judging the inter-specific relationshipof the various germpalsm materials in IlexL. and as one of the bases for the classificationon species-level. However, more molecular biological and morphological proofs were needed for determining the genetic differences of the various germplasm materials in the same species in IlexL. and for the classification of infra-specific taxa.