Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
Abstract:
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Highlights? A dimerization-dependent red fluorescent protein (ddRFP) was engineered ? ddRFP fluorogenesis results from modulation of pKa and quantum yield ? ddRFP-based biosensors exhibit reversible intensiometric responses ? ddRFP-based biosensors can detect Ca2+ dynamics and caspase-3 activity in live cells
