| 基于紫外光谱-支持向量机对STR的基因分型 |
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| 引用本文： | 徐容,吴再珍,郭莉萍,鲁辉,葛芝莉,谢洪平.基于紫外光谱-支持向量机对STR的基因分型[J].分析测试学报,2011,30(3):336-339. |
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| 作者姓名： | 徐容 吴再珍 郭莉萍 鲁辉 葛芝莉 谢洪平 |
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| 作者单位： | 1. 苏州大学医学部药学院,江苏苏州,215123
2. 重庆科技学院化学化工学院,重庆,401331 |
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| 基金项目： | 国家大学生创新性实验计划资助项目 |
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| 摘 要： | 利用紫外光谱结合支持向量机(SVM)模式识别原理建立了短串联重复序列(STR)的分型方法.以D5S818基因座的10-10、11-13和13-13三个基因型为研究对象,优化了包含该多态性位点的DNA片段的聚合酶链反应(PCR)的扩增条件和紫外光谱检测条件,获得了优化条件下的待测样本紫外光谱.以该光谱为识别变量,建立了该...
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| 关 键 词： | 短串联重复序列 紫外光谱 支持向量机 基因分型 |
Genotyping of STR Locus Based on Ultraviolet Spectroscopy and Support Vector Machine |
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| XU Rong,WU Zai-zhen,GUO Li-ping,LU Hui,GE Zhi-li,XIE Hong-ping.Genotyping of STR Locus Based on Ultraviolet Spectroscopy and Support Vector Machine[J].Journal of Instrumental Analysis,2011,30(3):336-339. |
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| Authors: | XU Rong WU Zai-zhen GUO Li-ping LU Hui GE Zhi-li XIE Hong-ping |
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| Institution: | XU Rong1,WU Zai-zhen1,GUO Li-ping2,LU Hui1,GE Zhi-li1,XIE Hong-ping1(1.College of Pharmaceutical Sciences,Soochow University,Suzhou215123,China,2.College of Chemistry and Chemical Engineering,Chongqing University of Science and Technology,Chongqing401331,China) |
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| Abstract: | An approach for genotyping of STR locus based on ultraviolet(UV) spectroscopy and support vector machine(SVM) was studied.Taking three different genotypes 10-10,11-13 and 13-13 of locus D5S818 as example,the conditions for PCR amplification and UV spectral detection on a DNA fragment containing the polymorphism sites were optimized.Under the optimal conditions,the ultraviolet spectra for the samples were obtained.Using these spectra as identifying variables,the SVM model of the three genotypes was establish... |
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| Keywords: | short tandem repeat ultraviolet spectroscopy support vector machine genotyping |
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