| Expression of Human Hepatitis B Virus Surface Antigen Gene in Transgenic Tobacco |
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| 作者姓名： | 刘玉乐 王晋芳 邱并生 赵淑珍 田波 |
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| 作者单位： | Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC |
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| 摘 要： | Expression of Human hepatitis B virus surface antigen (HBsAg) gene in plant was reported for the first time. The recombinant plasmid pRoKⅡ-HBsAg was constructed by inserting HBsAg gene into the downstream of CaMV 35S promoter of binary vector pRoKⅡ and then introduced into Agrobacterium tumefaciens LBA4404. The kanamycin-resistant plants were obtained by Agrobacterium-mediated transformation system. It was shown that HBsAg gene was expressed in transgenic tobacco plants and their progenies by ELISA. The spherical particles of ψ 22 nm in the leaf extract of trangenic tobacco were observed by immunosorbent electron microscopy.
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Expression of Human Hepatitis B Virus Surface Antigen Gene in Transgenic Tobacco |
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| LIU Yu-Le WANG Jin-Fang QIU Bing-ShengZHAO Shu-Zhen and TIAN Bo.Expression of Human Hepatitis B Virus Surface Antigen Gene in Transgenic Tobacco[J].Science in China(Chemistry),1994(1). |
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| Authors: | LIU Yu-Le WANG Jin-Fang QIU Bing-ShengZHAO Shu-Zhen and TIAN Bo |
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| Abstract: | |
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| Keywords: | hepatitis B virus surface antigen transgenic tobacco foreign gene expression |
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