| 脂肪酶仿生固定化及性质 |
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| 引用本文： | 王静云,马翠丽,包永明. 脂肪酶仿生固定化及性质[J]. 分子催化, 2011, 25(4): 341-347 |
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| 作者姓名： | 王静云 马翠丽 包永明 |
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| 作者单位： | 大连理工大学生命科学与技术学院,辽宁大连,116024 |
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| 基金项目： | 国家重点基础研究发展计划“973”项目(2009CB724706) |
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| 摘 要： | 将仿生钛化过程用于脂肪酶固定化,研究了该过程中工艺条件对脂肪酶固定化的影响及固定化脂肪酶的性质.结果表明：0.5 mL浓度8 mg/mL精蛋白诱导剂、0.5 mL浓度6 mg/mL脂肪酶与1 mL,0.25 mol/L钛前驱体（Ti-BALDH）在pH 7.5,0.05 mol/L磷酸盐缓冲液为反应介质的条件下,脂肪酶...
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| 关 键 词： | 仿生钛化 氧化钛 精蛋白 固定化脂肪酶 |
The immobilization of lipase by biomimetic mineralization and its properties |
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| Wang Jing-yun,Ma Cui-li and Bao Yong-ming. The immobilization of lipase by biomimetic mineralization and its properties[J]. Journal of Molecular Catalysis (China), 2011, 25(4): 341-347 |
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| Authors: | Wang Jing-yun Ma Cui-li Bao Yong-ming |
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| Affiliation: | WANG Jing-yun,MA Cui-li,BAO Yong-ming(School of life science & biotechnology,Dalian University of Technology,Dalian,116024,China) |
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| Abstract: | Biomimetic mineralization,which employs biological molecules to induce and drive the formation of inorganic oxides with excellent properties at room temperature,ambient pressure and neutral pH,provides a facile new method for enzyme immobilization.In the present study,for the first time,the biomimetic synthesis of titania was employed for the immobilization of lipase.The optimal encapsulation efficiency(70.1%) and activity recovery(20.3%) of lipase were achieved when 0.5 mL protamine solution of 8mg/mL and ... |
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| Keywords: | biomimetic mineralization titania protamine encapsulated lipase |
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