| 白藜芦醇与DNA相互作用的研究 |
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| 引用本文： | 赵明星,张淑芳,单瑞峰. 白藜芦醇与DNA相互作用的研究[J]. 分析科学学报, 2012, 28(3): 369-372 |
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| 作者姓名： | 赵明星 张淑芳 单瑞峰 |
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| 作者单位： | 曲阜师范大学化学与化工学院,山东曲阜,273165 |
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| 基金项目： | 国家自然科学基金，山东省自然科学基金 |
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| 摘 要： | 在pH=7.4的Tris-HCI缓冲溶液中,采用紫外光谱、荧光光谱及粘度法研究了白藜芦醇与小牛胸腺DNA(ctDNA)的相互作用。在生理条件下,白藜芦醇可以与ctDNA相互作用形成复合物,运用双倒数法求出了白藜芦醇与ctDNA相互作用的热力学参数。白藜芦醇能有效猝灭吖啶橙(AO)-DNA体系的荧光,猝灭方式为生成复合物的静态猝灭。粘度法实验证明ctDNA溶液的粘度随着白藜芦醇浓度的增加而增大。结果表明,白藜芦醇与DNA发生了较强的相互作用,其键合模式为经典的插入方式。
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| 关 键 词： | 白藜芦醇 小牛胸腺DNA 紫外光谱法 荧光光谱法 |
Studies on the Interaction of Resveratrol with Deoxyribonucleic Acid |
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| ZHAO Ming-xing , ZHANG Shu-fang , SHAN Rui-feng. Studies on the Interaction of Resveratrol with Deoxyribonucleic Acid[J]. Journal of Analytical Science, 2012, 28(3): 369-372 |
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| Authors: | ZHAO Ming-xing ZHANG Shu-fang SHAN Rui-feng |
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| Affiliation: | (College of Chemistry and Chemical Engineering,Qufu Normal University,Qufu273165) |
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| Abstract: | The interaction of resveratrol with calf thymus deoxyribonucleic acid(ctDNA)under physiological conditions(Tris-HCl buffer solutions,pH =7.4)was studied by UV spectroscopy,fluorescence spectroscopy and viscosity measurements.Results indicate that a complex of resveratrol and ctDNA was formed,and the thermodynamic parameters of the complex were calculated by a double reciprocal method.The fluorescence quenching mechanism of AO-ctDNA by using resveratrol was a static quenching type.By combination of viscosity determination results,we suggested that the interaction model between resveratrol and ctDNA be the intercalation bindings. |
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| Keywords: | Resveratrol Calf thymus DNA UV spectra Fluorescence spectra |
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