| Abstract: | Relationships between log k' values of polynuclear hydrocarbons and composition of water/methanol mixtures were determined for HPTLC on RP-18 silica and for HPLC using RP-2 silica. In spite of differences in the adsorbents used, a good correlation between HPLC and TLC parameters was found. In the TLC experiments, a sandwich tank with a modified solvent distributor was used. It has been reported that thin-layer chromatography is a good technique for the preliminary optimization of column chromatographic separations 1–5]. So far, the comparison of TLC and HPLC has been mainly restricted to silica as the adsorbent. Since TLC plates precoated with silanized adsorbents recently became commercially available, it seemed interesting to compare the chromatographic parameters obtained for the “reversed phase” systems with the two techniques (see also ref. 6). |
