Lactate Biosensor Based on Hydrotalcite‐Like Compounds: Performances and Application to Serum Samples |
| |
| Authors: | Irene Carpani Lorella Guadagnini Domenica Tonelli |
| |
| Institution: | Department of Physical and Inorganic Chemistry, University of Bologna, Viale Risorgimento 4, 40136?Bologna, INSTM, UdR Bologna, Italy |
| |
| Abstract: | A lactate biosensor based on lactate oxidase supported onto a hydrotalcite, electrochemically deposited on a platinum surface, was developed for the first time. For the best electrode configuration, a linear response up to 0.8 mM, with a limit of detection of 14 μM and a sensitivity of 91 mA M?1 cm?2, was obtained. The influence of some interferents due to the oxidation of hydrogen peroxide (at +0.35 V vs. SCE) was also studied. By controlling carefully the experimental conditions, the determination of lactate in a commercial serum sample in the presence of interferents was successfully accomplished. |
| |
| Keywords: | Lactate Biosensors Hydrotalcite Electrodeposition Bovine serum Enzymes |
|
|
