Simultaneous enantioseparation of cyproconazole,bromuconazole, and diniconazole enantiomers by CD‐modified MEKC |
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Authors: | Wan Aini Wan Ibrahim Susanti A Warno Hassan Y Aboul‐Enein Dadan Hermawan Mohd Marsin Sanagi |
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Institution: | 1. Separation Science Research Group, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, Malaysia;2. Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, Cairo Egypt |
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Abstract: | An efficient method for the simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers was developed by CD‐modified MEKC using a dual mixture of neutral CDs as chiral selector. Three neutral CDs namely hydroxypropyl‐β‐CD, hydroxypropyl‐γ‐CD, and γ‐CD were tested as chiral selectors at different concentrations ranging from 10, 20, 30 and 40 mM, but enantiomers of the studied fungicides were not completely separated. The best dual chiral recognition mode for the simultaneous separation of cyproconazole, bromuconazole, and diniconazole enantiomers was achieved with a mixture of 27 mM hydroxypropyl‐β‐CD and 3 mM hydroxypropyl‐γ‐CD in 25 mM phosphate buffer (pH 3.0) containing 40 mM SDS to which methanol‐acetonitrile (10%:5% v/v) was added as organic modifiers. The best separation was based on the appearance of 10 peaks simultaneously, with good resolution (Rs 1.1–15.9), and peak efficiency (N>200 000). Good repeatabilities in the migration time, peak area, and peak height were obtained in terms of RSD ranging from (0.72 to 1.06)%, (0.39 to 3.49)%, and (1.90 to 4.84)%, respectively. |
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Keywords: | CD‐modified MEKC Chiral triazole fungicides Dual CD Enantioseparation |
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