Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE‐ESI‐MS |
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| Authors: | Xingyu Zhu Kenichi Hatanaka Tatsuya Yamagata Toshinori Sato |
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| Institution: | 1. Department of Biosciences and Informatics, Keio University, Yokohama, Japan;2. Institute of Industrial Science, The University of Tokyo, Tokyo, Japan;3. Department of Life Sciences, Shenyang Pharmaceutical University, Shenyang, P. R. China |
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| Abstract: | A glycosphingolipid analogue (12‐azidododecyl β‐lactoside) as a saccharide primer has been shown to be useful for the synthesis of oligosaccharide libraries by mammalian cells. In the present study, CE‐ESI‐MS was employed to elucidate the structure of glycosphingolipid analogues derived from 12‐azidododecyl β‐lactoside (Lac‐C12N3) by mammalian cells. MDCK cells and COLO201 cells were cultured with Lac‐C12N3, and the glycosylated products secreted into the medium were collected and separated into acidic and neutral products by column chromatography. The acidic products could be directly analyzed by CE‐ESI‐MS, while the neutral products were converted to anionic derivatives via a reaction with propiolic acid. With this method, it was possible to analyze both acidic and neutral products glycosylated by MDCK cells and COLO201 cells at high sensitivity. |
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| Keywords: | CE‐ESI‐MS Neutral carbohydrate Saccharide primer |
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