Responsive Metal Complexes: A Click‐Based “Allosteric Scorpionate” Complex Permits the Detection of a Biological Recognition Event by EPR/ENDOR Spectroscopy
1. School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS (UK), Fax: (+44)?20‐7882‐7427;2. School of Chemistry, The University of Sydney, NSW 2006 (Australia), Fax: (+61)?2‐9351‐3329
Abstract:
Click to detect! Azamacrocyclic complexes with a triazole scorpion ligand may be easily assembled, as shown for a biotin‐functionalised cyclam derivative. Coordination of the triazole to the metal is perturbed by the binding of avidin to the pendant ligand (see scheme). This event can be sensitively detected with EPR and ENDOR spectroscopy, which confirm the loss of the axial triazole nitrogen donor upon avidin binding. This general strategy may have wide applications in imaging and therapeutics.
