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Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme‐Linked Immunosorbent Assay Employing Chicken Yolk Antibodies
Authors:Sona Krizkova  Pavlina Blahova  Johana Nakielna  Ivo Fabrik  Vojtech Adam  Tomas Eckschlager  Miroslava Beklova  Zdenka Svobodova  Vratislav Horak  Rene Kizek
Institution:1. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ‐613 00 Brno, Czech Republic;2. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ‐613?00 Brno, Czech Republic;3. Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University, V Uvalu 84, CZ‐150?06 Prague 5, Czech Republic;4. Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Palackeho 1?–?3, CZ‐612?42 Brno, Czech Republic;5. Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1‐3, CZ‐612?42 Brno, Czech Republic;6. Research Institute of Fish Culture and Hydrobiology, University of South Bohemia Ceske Budejovice, Zatisi 728/II, CZ‐389?25 Vodnany, Czech Republic;7. Laboratory of Tumour Biology, Department of Animal Embryology, Cell and Tissue Differentitation, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., CZ‐277?21 Libechov, Czech Republic
Abstract:In this study, two methods for metallothioneins (MT) determination in a biological sample were compared. Particularly, twenty five human and nine pig blood serum samples and liver and kidney samples from thirty five carps (Cyprinus carpio) were analyzed by enzyme‐linked immunosorbent assay and differential pulse voltammetry Brdicka's reaction. The results obtained by these two methods were in good agreement. For commercially available MT standard the correlation coefficient between the single concentrations signal height was 0.99. In biological samples the correlation coefficients were 0.90 for fish liver and kidney samples, 0.91 for pig blood serum and 0.93 for human blood serum.
Keywords:Metallothionein  Differential pulse voltammetry  ELISA  Chicken yolk antibodies  Human blood serum  Immunoassays  Antibodies
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