Discovery of New Natural Products by Intact‐Cell Mass Spectrometry and LC‐SPE‐NMR: Malbranpyrroles,Novel Polyketides from Thermophilic Fungus Malbranchea sulfurea |
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| Authors: | Yu‐Liang Yang Dr. Wen‐Ying Liao Wan‐Yun Liu Chih‐Chuang Liaw Dr. Chia‐Ning Shen Dr. Zih‐You Huang Shih‐Hsiung Wu Prof. Dr. |
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| Affiliation: | 1. Institute of Biological Chemistry, Academia Sinica, Taipei 115 (Taiwan), Fax: (+866)?2‐2653‐9142;2. Graduate Institute of Pharmaceutical Chemistry, College of Pharmacy, China Medical University, Taichung 404 (Taiwan);3. Genomics Research Center, Academia Sinica, Taipei 115 (Taiwan);4. Department of Life Sciences, National Chung‐Hsing University, Taichung 402 (Taiwan) |
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| Abstract: | Six photosensitive polyketides, malbranpyrroles A–F, were discovered from the thermophilic fungus Malbranchea sulfurea by using intact‐cell desorption/ionization on silicon mass (ICD‐MS) and LC‐SPE‐NMR. These two strategies facilitate the searching and structural determination of unstable natural products. The ICD‐MS indicated that only brown hyphae of M. sulfurea can produce malbranpyrroles. The biosynthetic pathway of malbranpyrroles was evidenced by 13C isotope precursors and amino acid feeding experiments. The cytotoxicity data revealed that the conformation of the conjugated system in malbranpyrroles does not affect cytotoxic potency against cancer cell lines. In addition, the chlorine atom was shown to be the pharmacophore for cytotoxicity. |
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| Keywords: | Malbranchea sulfurea mass spectrometry natural products NMR spectroscopy polyketides |
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