Reagentless Biosensor for Hydrogen Peroxide Based on the Immobilization of Hemoglobin in Platinum Nanoparticles Enhanced Poly(chloromethyl thiirane) Cross‐linked Chitosan Hybrid Film |
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| Authors: | Shanshan Jia Junjie Fei Tian Tian Fangqin Zhou |
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| Institution: | 1. College of Chemistry, Xiangtan University, Xiangtan, 411105, P.?R. China;2. Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Xiangtan University, Xiangtan, 411105, P.?R. China |
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| Abstract: | An unmediated hydrogen peroxide (H2O2) biosensor was prepared by co‐immobilizing hemoglobin (Hb) with platinum nanoparticles enhanced poly(chloromethyl thiirane) cross‐linked chitosan (CCCS‐PNs) hybrid film. CCCS could provide a biocompatible microenvironment for Hb and PNs could accelerate the electron transfer between Hb and the electrode. Spectroscopic analysis indicated that the immobilized Hb could maintain its native structure in the CCCS‐PNs hybrid film. Entrapped Hb exhibited direct electrochemistry for its heme Fe(III)/Fe(II) redox couples at ?0.396 V in the CCCS‐PNs hybrid film, as well as peroxidase‐like activity to the reduction of hydrogen peroxide without the aid of an electron mediator. |
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| Keywords: | Cross‐linked chitosan Platinum nanoparticles Hemoglobin Hydrogen peroxide Biosensor |
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