Nerve Agents Assay Using Cholinesterase Based Biosensor |
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| Authors: | Miroslav Pohanka Petr Dobes Lucie Drtinova Kamil Kuča |
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| Affiliation: | 1. Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic;2. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic;3. Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic;4. Department of Chemistry, Faculty of Sciences, J.?E. Purkinje University, Usti nad Labem, Czech Republic;5. Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic |
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| Abstract: | Electrochemical biosensor based on electric eel acetylcholinesterase (AChE) (EC 3.1.1.7) was performed for assay of nerve agents – tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by a strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created from acetylthiocholine (ATChCl) by AChE‐catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.88×10?10 M) and VX (8.51×10?10 M) after one‐minute assay. The biosensor was found long term stable at low as well as laboratory temperature. |
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| Keywords: | Organophophate Biosensor Acetylcholinesterase Sarin Soman VX Tabun Cyclosarin |
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