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Disposable Electrodes for Capacitive Immunosensor |
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| Authors: | Kosin Teeparuksapun Proespichaya Kanatharana Warakorn Limbut Chongdee Thammakhet Punnee Asawatreratanakul Bo Mattiasson Booncharoen Wongkittisuksa Chusak Limsakul Panote Thavarungkul |
| Affiliation: | 1. Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;2. Center for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;3. Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;4. Department of General Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;5. Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;6. Department of Biotechnology, Lund University, Box 124, 221?00 Lund, Sweden;7. Department of Electrical Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;8. Department of Physics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand |
| Abstract: | Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm×25.4 mm) and used in a label‐free immunosensor. Human serum albumin (HSA) and its antigen (anti‐HSA) were used as a model system. Electropolymerization of o‐phenylenediamine was used for the immobilization of anti‐HSA by covalent binding. A linear relationship was obtained in the range from 1.0×10?14 to 1.0×10?9 M with a limit of detection of 8.0×10?15 M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method. |
| Keywords: | Disposable electrode Thermal evaporation o‐Phenylenediamine Capacitive immunosensor Human serum albumin |