Photodynamic Activity of C70 Caged within Surface‐Cross‐Linked Liposomes |
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| Authors: | Atsushi Ikeda Dr Mai Nagano Motofusa Akiyama Dr Masashi Matsumoto Sayuri Ito Masaru Mukai Mineo Hashizume Dr Jun‐ichi Kikuchi Prof Kiyofumi Katagiri Dr Takuya Ogawa Dr Tatsuo Takeya Prof |
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| Institution: | 1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916‐5 Takayama, Ikoma, Nara 630‐0192 (Japan). Fax: (+81)?743‐72‐6099;2. Graduate School of Engineering, Nagoya University, Furo‐cho, Chikusa‐ku, Nagoya 464‐8603 (Japan).;3. Graduate School of Biological Science, Nara Institute of Science and Technology, 8916‐5 Takayama, Ikoma, Nara 630‐0192 (Japan). |
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| Abstract: | 70]Fullerene (C70) encapsulated into a surface‐cross‐linked liposome, a so‐called cerasome, was prepared by an exchange reaction incorporating C70?γ‐cyclodextrin complexes into lipid membranes. Fullerene exchange in a cerasome‐incorporated C70 (CIC70), as well as in a lipid‐membrane‐incorporated C70 (LMIC70), was completed within 1 min with stirring at 25 °C. CIC70 was more resistant to lysis than LMIC70 towards lysing agents such as surfactants. Furthermore, the photodynamic activity of CIC70 in HeLa cells was similar to that of LMIC70, indicating that C70 can act as a photosensitizing drug (PS) without release from cerasome membranes. Thus, in contrast with general drug‐delivery systems (DDSs), which require the drug to be released from the interior of liposomes, carriers for PSs for use in photodynamic therapy (PDT) do not necessarily need to release the drug. These results indicate that DDSs with high morphological stability can increase the residence time in blood and achieves tumor‐selective drug delivery by the enhanced permeability and retention (EPR) effect. |
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| Keywords: | cyclodextrins exchange reactions fullerenes lipid bilayers photodynamic activities |
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