Department of Chemistry and Konstanz Research School Chemical Biology (KoRS‐CB), University of Konstanz, Universit?tsstrasse 10, 78457 Konstanz (Germany), Fax: (+49)?7531‐885‐140 http://www.uni‐konstanz.de/FuF/chemie/jhartig
Abstract:
Fusions of a ribozyme and an aptamer of a natural riboswitch (thiamine pyrophosphate, TPP) are used to construct artificial thiamine‐dependent switches of gene expression. As J. S. Hartig et al. describe in their Communication on page 2715 ff. , insertion of these RNA elements into bacterial mRNAs allows translation of the message to be switched on or off. TPP triggers changes to the ribozyme‐mediated mRNA cleavage, resulting in liberation of the message for translational initiation.
