Platform for Highly Sensitive Alkaline Phosphatase‐Based Immunosensors Using 1‐Naphthyl Phosphate and an Avidin‐Modified Indium Tin Oxide Electrode |
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| Authors: | Md. Abdul Aziz Kyungmin Jo Md. Abdul Qaium Chan‐Hwa Huh In Seok Hong Haesik Yang |
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| Affiliation: | 1. Department of Chemistry, Pusan National University, Busan 609‐735, Korea;2. Department of Chemistry, Kongju National University, Kongju 314‐701, Korea |
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| Abstract: | We report a versatile platform for highly sensitive alkaline phosphatase (ALP)‐based electrochemical biosensors that uses an avidin‐modified indium tin oxide (ITO) electrode as a sensing electrode and 1‐naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1‐naphthol (ALP product) on the ITO electrodes allow a high signal‐to‐background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL. |
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| Keywords: | Immunosensor Alkaline phosphatase 1‐Naphthyl phosphate Avidin Nonspecific binding Biosensors Enzymes |
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