A Third‐Generation Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Covalently Immobilized on Electrografted Organic Film on Screen‐Printed Carbon Electrode |
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| Authors: | Abd‐Elgawad Radi Xavier Muñoz‐Berbel Montserrat Cortina‐Puig Jean‐Louis Marty |
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| Affiliation: | 1. Department of Chemistry, Faculty of Science, Mansoura University, 34517?Dumyat, Egypt;2. Centre Nacional de Microelectrònica (IMB‐CSIC), Esfera UAB, Campus Univ. Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;3. BIOMEM, Université de Perpignan, 52 Avenue Paul Alduy, 66860?Perpignan Cedex, France |
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| Abstract: | A new convenient strategy to fabricate a third‐generation hydrogen peroxide biosensor was described. The screen‐printed carbon electrode (SPCE) was first modified with a layer of 4‐nitrophenyl assembled from the 4‐nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP‐modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long‐term stability. |
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| Keywords: | Diazonium salt Horseradish peroxidase Direct electrochemistry Hydrogen peroxide Screen‐printed carbon electrode Biosensors Enzymes |
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