Direct Electrochemistry of Hemoglobin Immobilized on Colloidal Gold‐Hydroxyapatite Nanocomposite for Electrocatalytic Detection of Hydrogen Peroxide |
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Authors: | Juan You Weiping Ding Shijia Ding Huangxian Ju |
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Institution: | 1. Key Laboratory of Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.?R. China;2. Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P.?R. China |
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Abstract: | A novel nanocomposite of colloidal gold (GNPs) and hydroxyapatite nanotubes (Hap) was prepared for immobilization of a redox protein, hemoglobin (Hb), on glassy carbon electrode. The immobilized Hb showed fast direct electron transfer and excellent electrocatalytic behavior toward reduction of hydrogen peroxide. A synergic effect between GNPs and Hap for accelerating the surface electron transfer of Hb was observed, which led to a pair of redox peaks with a formal potential of (?340±2) mV at pH 7.0, and a new biosensor for hydrogen peroxide with a linear range from 0.5 to 25 μM and a limit of detection of 0.2 μM at 3σ. Owing to the good biocompatibility of the nanocomposite, the biosensor exhibited good stability and acceptable reproducibility. The as‐prepared nanocomposite film provided a good matrix for protein immobilization and biosensor preparation. |
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Keywords: | Hydroxyapatite Gold nanoparticles Nanocomposite Hemoglobin Direct electrochemisty Biosensor |
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