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Synthesis of a Sialic Acid Containing Complex‐Type N‐Glycan on a Solid Support |
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| Authors: | Katsunori Tanaka Dr. Yohei Fujii Hiroomi Tokimoto Dr. Yasutaka Mori Shin‐ichi Tanaka Dr. Guang‐ming Bao Eric R. O. Siwu Dr. Aiko Nakayabu Koichi Fukase Prof. Dr. |
| Affiliation: | Department of Chemistry, Graduate School of Science, Osaka University, 1‐1 Machikaneyama‐cho, Toyonaka‐shi, Osaka 560‐0043 (Japan), Fax: (+81)?6‐6850‐5419 |
| Abstract: | On solid ground : A new solid‐phase synthesis of N‐linked glycans featuring 1) highly stereoselective β‐mannosylation and microfluidic α‐sialylation and 2) efficient glycosylation of the N‐phenyltrifluoroacetimidate units on JandaJel resin is reported. Reagent concentration effects by a fluorous solvent are effectively applied, and the use of these methods results in the first synthesis of a sialic acid containing complex‐type N‐glycan on a solid support.  |
| Keywords: | carbohydrates glycoproteins glycosylation microfluidics solid‐phase synthesis |