Determination of 5‐Aminosalicylic Acid by Catalase‐Peroxidase Based Biosensor |
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Authors: | Alper Akkaya Cagri Altug Nurdan Kasikara Pazarlioglu Erhan Dinckaya |
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Affiliation: | Ege University, Faculty of Science, Biochemistry Department, Bornova‐Izmir 35100, Turkey |
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Abstract: | 5‐Aminosalicylic acid is an antiinflammatory drug used to treat inflammation of the digestive tract (Crohn's disease) and mild to moderate ulcerative‐colitis. 5‐Aminosalicylic acid is a bowel‐specific aminosalicylate drug. It was developed an amperometric biosensor for determination of 5‐aminosalicylic acid concentration and measurement technique is based on substrate‐competition. The biosensor is more suitable especially for routine 5‐aminosalicylic acid analysis because it is simple to construct and sensitive, specific and does not require any expensive apparatus. This enzyme based biosensor was made with a couple of enzymes which uses the same substrate. The electrode was developed to determine measurement conditions and also characterized. |
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Keywords: | Bienzyme system Catalase Peroxidase 5‐Aminosalicylic acid Biosensors Medicinal chemistry |
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