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The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid |
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| Authors: | Hakan Karadeniz Guliz Armagan Arzum Erdem Ezgi Turunc Ayfer Caliskan Lutfiye Kanit Ayfer Yalcin |
| Institution: | 1. Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100?Bornova, Izmir, Turkey;2. Ege University, Faculty of Pharmacy, Biochemistry Department, 35100 Bornova, Izmir, Turkey;3. Ege University, Faculty of Medicine, Center for Brain Research, 35100?Bornova, Izmir, Turkey |
| Abstract: | A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded DNA and genomic DNA was herein determined in in vitro and in vivo conditions using; electrochemical assay and agarose gel electrophoresis. The changes in guanine signal were detected as an indicator of DNA damage in genomic DNA samples isolated from 1 or 10 mg/kg KA‐treated animals. The decreased levels of guanine signal were found as 29% and 33% by 1 and 10 mg/kg KA treatment when compared to controls, respectively. The results of gel electrophoresis confirmed DNA damage obtained in identical samples by electrochemical method. |
| Keywords: | DNA damage Kainic acid Electrochemical sensor technology Agarose gel electrophoresis Guanine signal DNA damage Electrophoresis |