A Novel Hydrogen Peroxide Biosensor Based on the Synergistic Effect of Gold‐Platinum Alloy Nanoparticles/Polyaniline Nanotube/Chitosan Nanocomposite Membrane |
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Authors: | Xueliang Wang Tao Yang Yuanyuan Feng Kui Jiao Guicun Li |
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Affiliation: | 1. Key Laboratory of Eco‐chemical Engineering (Ministry of Education), College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.?R. China;2. Key Laboratory of Nanostructured Materials, Qingdao University of Science and Technology, Qingdao 266042, P.?R. China |
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Abstract: | Based on the immobilization of horseradish peroxidase (HRP) in chitosan(CS) on a glassy carbon electrode (GCE) modified with the Au‐Pt alloy nanoparticles (NPs) / polyaniline nanotube (nanoPAN) nanocomposite film, a novel hydrogen peroxide biosensor was constructed. The modified processes of GCE were monitored by cyclic voltammetry and electrochemical impedance spectroscopy. Au‐PtNPs/nanoPAN/CS had a better synergistic electrochemical effect than did AuNPs/nanoPAN/CS or PtNPs/nanoPAN/CS. The amperometric response of the biosensor towards H2O2 was investigated by successively adding aliquots of H2O2 to a continuous stirring phosphate buffer solution under the optimized conditions. Because Au‐PtNPs have unique catalytic properties and good biocompatibility, and especially Au‐PtNPs and nanoPAN have synergistic augmentation for facilitating electron‐transfer, the biosensor displayed a fast response time (<2 s) and broad linear response to H2O2 in the range from 1.0 to 2200 μmol L?1 with a relatively low detection limit of 0.5 μmol L?1 at 3 times the background noise. Moreover, the biosensor can be applied in practical analysis and exhibited high sensitivity, good reproducibility, and long‐term stability. |
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Keywords: | Horseradish peroxidase Polyaniline nanotube Gold‐platinum alloy nanoparticles Hydrogen peroxide Synergistic effects Biosensors |
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