微粒形体特征概率分布函数的光散射频谱分析法

针对微粒(包括生物微粒)光散射测量中微粒形体对测量结果的影响以及形体分布不能实时测量的问题,本文根据光散射测量原理,从微粒群的统计性出发,对散射脉冲的脉宽、脉峰分布及其与微粒大小和形体分布物理动态特征的相互关系进行了系统的分析,得到了微粒形体、微粒大小分布与其散射谱分布的特征关系,提出了等值概率面积确定法则,并据此建立了一种实时由散射谱一体分析获取微粒形体特征分布的方法,得到了较好的实验验证,由此可有效地修正因微粒形体变化而对测量精度的影响,从而为粒度分析仪以及以光散射原理为测量理论的流式细胞仪技术的提升提供了有力的基础。

Analytical Method of Light Scattering Compose for the Probability Distribution Function of Particles Shape

In this paper,aim at the question of measuring results infected by particles' shape and real time measurement of it in the light scattering measurement.Fist,the scattering pulse width and peak distribution and the relation of particles' size and shape characters are systems analyzed based on the light scattering and statistical theory.In addition,a theorem of equivalence probability area is setup and a method of obtaining the probability distribution function of shape particles in real time is built.At last,the theories are validated in the experiments.These modify the question and offer a powerful foundation for FCA and PC in the measuring theory and its technology.