Determination of lansoprazole and its metabolites in plasma by high-performance liquid chromatography using a loop column. |
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| Authors: | B D Landes G Miscoria B Flouvat |
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| Affiliation: | Laboratoire de Toxicologie et de Pharmacocinétique, H?pital Ambroise Paré, Boulogne Billancourt, France. |
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| Abstract: | A high-performance liquid chromatographic method for the simultaneous determination of lansoprazole, a new proton pump inhibitor, and its metabolites in human plasma is described. Lansoprazole, its metabolites and an internal standard were extracted with tert.-butyl methyl ether. Samples were injected using an automatic injector via a loop column, and separation was obtained using a reversed-phase column under isocratic conditions. The absorbance was monitored at 285 and 303 nm. The quantification limit was 2 ng/ml for lansoprazole and 3 or 5 ng/ml for the metabolites. No endogenous compounds were found to interfere. The mean overall recovery was between 75 and 95% for lansoprazole and its metabolites. This method is suitable for pharmacokinetic studies. |
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