Enhancing the stability of kinesin motors for microscale transport applications |
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Authors: | Uppalapati Maruti Huang Ying-Ming Jackson Thomas Nelson Hancock William Olaf |
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Affiliation: | Department of Bioengineering, Penn State University, 229 Hallowell Building, University Park, PA 16802, USA. |
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Abstract: | Biomolecular motors, such as kinesins, have great potential for micro-actuation and micro- or nanoscale active transport when integrated into microscale devices. However, the stability and limited shelf life of these motor proteins and their associated protein filaments is a barrier to their implementation. Here we demonstrate that freeze-drying or critical point-drying kinesins adsorbed to glass surfaces extends their lifetime from days to more than four months. Further, photoresist deposition and removal can be carried out on these motor-adsorbed surfaces without loss of motor function. The methods developed here are an important step towards realizing the integration of biological motors into practical devices, and these approaches can be extended to patterning and preserving other proteins immobilized on surfaces. |
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