| 全波长扫描式多功能读数仪-分光光度法测定土壤中的无机氮 |
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| 引用本文： | 宋书会,王汝健,辛宇,张金尧,汪洪. 全波长扫描式多功能读数仪-分光光度法测定土壤中的无机氮[J]. 中国无机分析化学, 2019, 9(2): 35-41 |
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| 作者姓名： | 宋书会 王汝健 辛宇 张金尧 汪洪 |
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| 作者单位： | 中国农业科学院农业资源与农业区划研究所国家化肥质量监督检验中心(北京)农业农村部农产品质量安全肥料源性因子风险评估实验室(北京) |
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| 摘 要： | 快速准确测定土壤中铵态氮、硝态氮含量对监测土壤肥力水平和生态环境,指导作物氮肥施用非常重要。选择30份土样,利用全波长扫描式多功能读数仪(酶标仪)结合靛酚蓝分光光度法、硫酸肼还原法测定土壤中铵态氮和硝态氮含量,探讨利用酶标仪测定土壤无机氮含量的可行性。结果显示,利用酶标仪测定土壤铵态氮、硝态氮含量与连续流动分析仪测定结果之间无明显差异,彼此间呈显著线性相关。铵态氮回归直线方程为Y(连续流动分析仪-NH_4~+-N)=0.997 6 X(酶标仪-NH_4~+-N)-0.012 3,相关系数R=0.961 9(n=30,P0.01);硝态氮回归方程为Y(连续流动分析仪-NO_3~--N)=0.959 3 X(酶标仪-NO_3~--N)+0.021 9,相关系数R=0.964 0(n=30,P0.01)。酶标仪测定铵态氮回收率在96.2%～108%,相对标准偏差在10%以内;硝态氮测定回收率为94.9%～110%,且相对标准偏差在5%以内,酶标仪测定土壤铵态氮和硝态氮方法检出限分别为0.068mg/L和0.028mg/L。酶标仪测定土壤无机氮速度快,精密度、准确度较高,消耗试剂少,可用于大批量土壤浸提液中铵态氮和硝态氮含量的快速分析。
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| 关 键 词： | 铵态氮 硝态氮 酶标仪 土壤 |
| 收稿时间： | 2018-11-14 |
| 修稿时间： | 2018-12-11 |
Rapid determination of soil inorganic nitrogen by a microplate reader with colorimetric method |
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| SONG Shuhui,WANG Rujian,XIN Yu,ZHANG Jinyao and WANG Hong. Rapid determination of soil inorganic nitrogen by a microplate reader with colorimetric method[J]. Chinese Journal of Inorganic Analytical Chemistry, 2019, 9(2): 35-41 |
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| Authors: | SONG Shuhui WANG Rujian XIN Yu ZHANG Jinyao WANG Hong |
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| Affiliation: | Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing,Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing,Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing,Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing,Institute of Agriculture Resources and Regional Planning,China National Center for Quality Supervision and Test of Chemical FertilizersBeijing,Laboratory of Quality and Safety Risk Assessment for Agricultural Products from Fertilizer Sources,Ministry of Agriculture and Rural Affairs Beijing |
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| Abstract: | |
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| Keywords: | ammonium nitrogen nitrate nitrogen microplate reader soil |
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